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Decontamination Process Validation
Aseptic Sampling - What is Best Practice?

The last few months have made us all more aware of how often we touch our faces, and the importance of masks and PPE, but why is ensuring good aseptic technique important for water sampling, and what is best practice?

The answer to the first question is quite straightforward – if a water bottle or sample is compromised by microorganisms on your hands or improperly donned PPE (putting on gloves and then picking up a pen, or typing something on your laptop is very easy to do if you are not thinking about it), the sample may result in a false positive which no-one wants!


Unless there are specific instructions for a machine from the manufacturer, you should follow the following generic instructions:

How to Collect an Endoscopy Water Sample

  1. Apply Apron.
  1. 2. Apply Gloves.

3. Disinfect the point of contact thoroughly with an IPA wipe.

4. Draw off sample of water (this will be machine specific).

5. Fill all bottles provided to correct levels.

How to Collect an RO Plant/Sample Point

RO 1
RO 2
RO 4
RO 5
RO 8

1. Apply Gloves. (if taking multiple samples, use alcohol wipes contained in the PPE kit to disinfect gloves or change gloves

2. Disinfect the point of contact thoroughly with IPA spray/wipes.

3. Turn sample point on for initial flush. (this will be machine specific).

4. Fill all bottles provided to correct levels.

5.  Dispose of any excess water.

Certain tests have a stability time of 24 hours from sampling, and therefore the samples must be obtained so that the time between the sample being taken and the sample being testing is not greater than 24 hours – to find out more watch out for a future exciting installment!

It is critical to follow aseptic sampling techniques to reduce the risk of sample contamination.

If you would like more information on water sampling please call +44 (0) 114 354 0136 or email laboratory@isopharm.co.uk